perm buffer Search Results


flowx foxp3 fixation permeabilization buffer kit  (R&D Systems)
94
R&D Systems flowx foxp3 fixation permeabilization buffer kit
Flowx Foxp3 Fixation Permeabilization Buffer Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/perm+buffer/pmc05684192-243-16-23?v=R%26D+Systems
Average 94 stars, based on 1 article reviews
flowx foxp3 fixation permeabilization buffer kit - by Bioz Stars, 2026-08
94/100 stars
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1x foxp3 transcription factor fixation buffer  (R&D Systems)
92
R&D Systems 1x foxp3 transcription factor fixation buffer
1x Foxp3 Transcription Factor Fixation Buffer, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/perm+buffer/10__21608_slash_ejmr__2023__188321__1326-49-20-25?v=R%26D+Systems
Average 92 stars, based on 1 article reviews
1x foxp3 transcription factor fixation buffer - by Bioz Stars, 2026-08
92/100 stars
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maxpar barcode perm buffer  (fluidigm)
95
fluidigm maxpar barcode perm buffer
Maxpar Barcode Perm Buffer, supplied by fluidigm, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/perm+buffer/pmc08965896-138-4-8?v=fluidigm
Average 95 stars, based on 1 article reviews
maxpar barcode perm buffer - by Bioz Stars, 2026-08
95/100 stars
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maxpar fix  (fluidigm)
95
fluidigm maxpar fix
Maxpar Fix, supplied by fluidigm, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/perm+buffer/pm38480879-932-4-16?v=fluidigm
Average 95 stars, based on 1 article reviews
maxpar fix - by Bioz Stars, 2026-08
95/100 stars
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maxpar perm s buffer  (fluidigm)
93
fluidigm maxpar perm s buffer
Maxpar Perm S Buffer, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/perm+buffer/pmc10229197-153-27-30?v=fluidigm
Average 93 stars, based on 1 article reviews
maxpar perm s buffer - by Bioz Stars, 2026-08
93/100 stars
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flow cytometry perm buffer  (Cytek Biosciences)
97
Cytek Biosciences flow cytometry perm buffer
A) Flow <t>cytometry</t> gating of spike-specific (S+) and RBD-specific (RBD+) B cells. B) Phenotype of non-RBD-specific (S+RBD-) and RBD-specific (S+RBD+) B cells, showing the median percentage among each group. Graphs with individual data points for each B cell subset are presented in Figure S2 . C) Percentage of antigen-experienced spike-specific B cells in individuals who have never been exposed to SARS-CoV-2 (control) or who recovered from non-severe or severe disease. D) Percentage of spike-specific B cells that recognized RBD. E) Percentage of class-switched spike-specific B cells that express IgM (left), IgG (middle), or IgA (right). F) Plasma IgM (left) and IgG (right) titers against spike and RBD. Note that the range of the Y axes are different between IgM and IgG. In panel C, results are shown for individuals who recovered from non-severe COVID-19 (n = 8) and severe COVID-19 (n = 5). Only seven individuals with non-severe COVID-19 were included in panels B, D, and E, due to the low number of spike-specific B cells detected in the individual marked with # in panel C.
Flow Cytometry Perm Buffer, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/perm+buffer/bio_rxiv__2021__09__24__461732-196-32-17?v=Cytek+Biosciences
Average 97 stars, based on 1 article reviews
flow cytometry perm buffer - by Bioz Stars, 2026-08
97/100 stars
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perm/wash buffer 10x  (Becton Dickinson)
90
Becton Dickinson perm/wash buffer 10x
A) Flow <t>cytometry</t> gating of spike-specific (S+) and RBD-specific (RBD+) B cells. B) Phenotype of non-RBD-specific (S+RBD-) and RBD-specific (S+RBD+) B cells, showing the median percentage among each group. Graphs with individual data points for each B cell subset are presented in Figure S2 . C) Percentage of antigen-experienced spike-specific B cells in individuals who have never been exposed to SARS-CoV-2 (control) or who recovered from non-severe or severe disease. D) Percentage of spike-specific B cells that recognized RBD. E) Percentage of class-switched spike-specific B cells that express IgM (left), IgG (middle), or IgA (right). F) Plasma IgM (left) and IgG (right) titers against spike and RBD. Note that the range of the Y axes are different between IgM and IgG. In panel C, results are shown for individuals who recovered from non-severe COVID-19 (n = 8) and severe COVID-19 (n = 5). Only seven individuals with non-severe COVID-19 were included in panels B, D, and E, due to the low number of spike-specific B cells detected in the individual marked with # in panel C.
Perm/Wash Buffer 10x, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/perm+buffer/pm36302778-207-6-10?v=Becton+Dickinson
Average 90 stars, based on 1 article reviews
perm/wash buffer 10x - by Bioz Stars, 2026-08
90/100 stars
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perm/wash buffer #554723  (Becton Dickinson)
90
Becton Dickinson perm/wash buffer #554723
A) Flow <t>cytometry</t> gating of spike-specific (S+) and RBD-specific (RBD+) B cells. B) Phenotype of non-RBD-specific (S+RBD-) and RBD-specific (S+RBD+) B cells, showing the median percentage among each group. Graphs with individual data points for each B cell subset are presented in Figure S2 . C) Percentage of antigen-experienced spike-specific B cells in individuals who have never been exposed to SARS-CoV-2 (control) or who recovered from non-severe or severe disease. D) Percentage of spike-specific B cells that recognized RBD. E) Percentage of class-switched spike-specific B cells that express IgM (left), IgG (middle), or IgA (right). F) Plasma IgM (left) and IgG (right) titers against spike and RBD. Note that the range of the Y axes are different between IgM and IgG. In panel C, results are shown for individuals who recovered from non-severe COVID-19 (n = 8) and severe COVID-19 (n = 5). Only seven individuals with non-severe COVID-19 were included in panels B, D, and E, due to the low number of spike-specific B cells detected in the individual marked with # in panel C.
Perm/Wash Buffer #554723, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/perm+buffer/10__1161_slash_jaha__117__006949-85-9-9?v=Becton+Dickinson
Average 90 stars, based on 1 article reviews
perm/wash buffer #554723 - by Bioz Stars, 2026-08
90/100 stars
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perm buffer  (Becton Dickinson)
90
Becton Dickinson perm buffer
A) Flow <t>cytometry</t> gating of spike-specific (S+) and RBD-specific (RBD+) B cells. B) Phenotype of non-RBD-specific (S+RBD-) and RBD-specific (S+RBD+) B cells, showing the median percentage among each group. Graphs with individual data points for each B cell subset are presented in Figure S2 . C) Percentage of antigen-experienced spike-specific B cells in individuals who have never been exposed to SARS-CoV-2 (control) or who recovered from non-severe or severe disease. D) Percentage of spike-specific B cells that recognized RBD. E) Percentage of class-switched spike-specific B cells that express IgM (left), IgG (middle), or IgA (right). F) Plasma IgM (left) and IgG (right) titers against spike and RBD. Note that the range of the Y axes are different between IgM and IgG. In panel C, results are shown for individuals who recovered from non-severe COVID-19 (n = 8) and severe COVID-19 (n = 5). Only seven individuals with non-severe COVID-19 were included in panels B, D, and E, due to the low number of spike-specific B cells detected in the individual marked with # in panel C.
Perm Buffer, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/perm+buffer/pm19903659-92-5-7?v=Becton+Dickinson
Average 90 stars, based on 1 article reviews
perm buffer - by Bioz Stars, 2026-08
90/100 stars
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perm/washtm buffer 10  (Becton Dickinson)
90
Becton Dickinson perm/washtm buffer 10
A) Flow <t>cytometry</t> gating of spike-specific (S+) and RBD-specific (RBD+) B cells. B) Phenotype of non-RBD-specific (S+RBD-) and RBD-specific (S+RBD+) B cells, showing the median percentage among each group. Graphs with individual data points for each B cell subset are presented in Figure S2 . C) Percentage of antigen-experienced spike-specific B cells in individuals who have never been exposed to SARS-CoV-2 (control) or who recovered from non-severe or severe disease. D) Percentage of spike-specific B cells that recognized RBD. E) Percentage of class-switched spike-specific B cells that express IgM (left), IgG (middle), or IgA (right). F) Plasma IgM (left) and IgG (right) titers against spike and RBD. Note that the range of the Y axes are different between IgM and IgG. In panel C, results are shown for individuals who recovered from non-severe COVID-19 (n = 8) and severe COVID-19 (n = 5). Only seven individuals with non-severe COVID-19 were included in panels B, D, and E, due to the low number of spike-specific B cells detected in the individual marked with # in panel C.
Perm/Washtm Buffer 10, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/perm+buffer/us11498944-1611-6-9?v=Becton+Dickinson
Average 90 stars, based on 1 article reviews
perm/washtm buffer 10 - by Bioz Stars, 2026-08
90/100 stars
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1× transcription factor fix/perm buffer  (Becton Dickinson)
90
Becton Dickinson 1× transcription factor fix/perm buffer
A) Flow <t>cytometry</t> gating of spike-specific (S+) and RBD-specific (RBD+) B cells. B) Phenotype of non-RBD-specific (S+RBD-) and RBD-specific (S+RBD+) B cells, showing the median percentage among each group. Graphs with individual data points for each B cell subset are presented in Figure S2 . C) Percentage of antigen-experienced spike-specific B cells in individuals who have never been exposed to SARS-CoV-2 (control) or who recovered from non-severe or severe disease. D) Percentage of spike-specific B cells that recognized RBD. E) Percentage of class-switched spike-specific B cells that express IgM (left), IgG (middle), or IgA (right). F) Plasma IgM (left) and IgG (right) titers against spike and RBD. Note that the range of the Y axes are different between IgM and IgG. In panel C, results are shown for individuals who recovered from non-severe COVID-19 (n = 8) and severe COVID-19 (n = 5). Only seven individuals with non-severe COVID-19 were included in panels B, D, and E, due to the low number of spike-specific B cells detected in the individual marked with # in panel C.
1× Transcription Factor Fix/Perm Buffer, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/perm+buffer/pmc07925174-524-10-12?v=Becton+Dickinson
Average 90 stars, based on 1 article reviews
1× transcription factor fix/perm buffer - by Bioz Stars, 2026-08
90/100 stars
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perm buffer iii bd 558050  (Becton Dickinson)
90
Becton Dickinson perm buffer iii bd 558050
A) Flow <t>cytometry</t> gating of spike-specific (S+) and RBD-specific (RBD+) B cells. B) Phenotype of non-RBD-specific (S+RBD-) and RBD-specific (S+RBD+) B cells, showing the median percentage among each group. Graphs with individual data points for each B cell subset are presented in Figure S2 . C) Percentage of antigen-experienced spike-specific B cells in individuals who have never been exposed to SARS-CoV-2 (control) or who recovered from non-severe or severe disease. D) Percentage of spike-specific B cells that recognized RBD. E) Percentage of class-switched spike-specific B cells that express IgM (left), IgG (middle), or IgA (right). F) Plasma IgM (left) and IgG (right) titers against spike and RBD. Note that the range of the Y axes are different between IgM and IgG. In panel C, results are shown for individuals who recovered from non-severe COVID-19 (n = 8) and severe COVID-19 (n = 5). Only seven individuals with non-severe COVID-19 were included in panels B, D, and E, due to the low number of spike-specific B cells detected in the individual marked with # in panel C.
Perm Buffer Iii Bd 558050, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/perm+buffer/pmc11953300-340-9-12?v=Becton+Dickinson
Average 90 stars, based on 1 article reviews
perm buffer iii bd 558050 - by Bioz Stars, 2026-08
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Image Search Results


A) Flow cytometry gating of spike-specific (S+) and RBD-specific (RBD+) B cells. B) Phenotype of non-RBD-specific (S+RBD-) and RBD-specific (S+RBD+) B cells, showing the median percentage among each group. Graphs with individual data points for each B cell subset are presented in Figure S2 . C) Percentage of antigen-experienced spike-specific B cells in individuals who have never been exposed to SARS-CoV-2 (control) or who recovered from non-severe or severe disease. D) Percentage of spike-specific B cells that recognized RBD. E) Percentage of class-switched spike-specific B cells that express IgM (left), IgG (middle), or IgA (right). F) Plasma IgM (left) and IgG (right) titers against spike and RBD. Note that the range of the Y axes are different between IgM and IgG. In panel C, results are shown for individuals who recovered from non-severe COVID-19 (n = 8) and severe COVID-19 (n = 5). Only seven individuals with non-severe COVID-19 were included in panels B, D, and E, due to the low number of spike-specific B cells detected in the individual marked with # in panel C.

Journal: bioRxiv

Article Title: SARS-CoV-2 spike-specific memory B cells express markers of durable immunity after non-severe COVID-19 but not after severe disease

doi: 10.1101/2021.09.24.461732

Figure Lengend Snippet: A) Flow cytometry gating of spike-specific (S+) and RBD-specific (RBD+) B cells. B) Phenotype of non-RBD-specific (S+RBD-) and RBD-specific (S+RBD+) B cells, showing the median percentage among each group. Graphs with individual data points for each B cell subset are presented in Figure S2 . C) Percentage of antigen-experienced spike-specific B cells in individuals who have never been exposed to SARS-CoV-2 (control) or who recovered from non-severe or severe disease. D) Percentage of spike-specific B cells that recognized RBD. E) Percentage of class-switched spike-specific B cells that express IgM (left), IgG (middle), or IgA (right). F) Plasma IgM (left) and IgG (right) titers against spike and RBD. Note that the range of the Y axes are different between IgM and IgG. In panel C, results are shown for individuals who recovered from non-severe COVID-19 (n = 8) and severe COVID-19 (n = 5). Only seven individuals with non-severe COVID-19 were included in panels B, D, and E, due to the low number of spike-specific B cells detected in the individual marked with # in panel C.

Article Snippet: After the incubation, the cells were washed twice with 3 ml of 1× Flow Cytometry Perm Buffer (Tonbo) and resuspended in intracellular marker antibody cocktail ( Table S2 ) diluted in 1× Flow Cytometry Perm Buffer.

Techniques: Flow Cytometry, Control, Clinical Proteomics